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基于CRISPR的一步法黑腹果蝇内源性基因标记策略

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

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基于CRISPR的一步法黑腹果蝇内源性基因标记策略

DOI:

10.3791/64729-v

•

07:23 min

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January 26, 2024

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Yangbo Xiao*¹, Ye Yuan*², Swathi Yadlapalli^2,3

¹Department of Cell and Developmental Biology,University of Michigan, ²Cellular and Molecular Biology Program,University of Michigan, ³Michigan Neuroscience Institute Affiliate,University of Michigan

Capitoli

00:00Introduction
00:30Design and Construction of Gene Editing Reagents
06:51Results: Analyzing PERIOD-mNeonGreen Protein Expression in Clock Neurons
07:10Conclusion

Summary

Traduzione automatica

在这里，我们提出了一种简化的果蝇内源性基因标记方案，该方案利用基于PCR的技术对成功的基因修饰进行无标记鉴定，从而促进了稳定的敲入系的开发。

Tags

CRISPR Gene Tagging Drosophila Melanogaster Homologous Recombination Fluorescent Protein Knock-in SgRNA Donor Vector Cloning Plasmid Sanger Sequencing

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