Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Mathilde Beaufils; Amandine Tourel; Anne Petiot; Nicole B. Halmai; Dave J. Segal; John Rendu; Isabelle Marty

doi:10.3791/64114

Rivista

/

Genetica

/

Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing

JoVE Journal
Genetica

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Genetica

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Please note that all translations are automatically generated. Click here for the English version.

Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing

3,726 Views

•

10:12 min

•

June 16, 2022

DOI:

10.3791/64114-v

DOI:

10.3791/64114-v

•

10:12 min

June 16, 2022

•

2 Views

Mathilde Beaufils¹, Amandine Tourel¹, Anne Petiot¹, Nicole B. Halmai², Dave J. Segal², John Rendu¹, Isabelle Marty¹

¹University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, ²Genome Center,University of California, Davis

Summary

Das Protokoll beschreibt, wie Knock-out-Myoblasten mit CRISPR/Cas9 erzeugt werden können, beginnend mit dem Design von Guide-RNAs bis hin zur zellulären Klonierung und Charakterisierung der Knock-out-Klone.

Explore More Videos

CRISPR/Cas9 Gene Editing Knock-out Muscle Cells Lentivirus IPS Cells Guide RNA PCR Agarose Gel Restriction Enzymes Plasmid Cloning

Read Article

Entwicklung von Knock-out-Muskelzelllinien mittels Lentivirus-vermittelter CRISPR/Cas9-Gen-Editing

Summary

Automatically generated

Explore More Videos