Rivista
/
/
巴西黑线虫感染模型中产生IL-9的淋巴细胞研究策略
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model
DOI:

08:38 min

March 03, 2023

, , , , ,

Capitoli

  • 00:05Introduction
  • 01:03Isolation of Mediastinal Lymph Nodes, Lungs, Mesenteric Lymph Node Chains, and Small Intestine
  • 02:37Preparation of Single-Cell Suspensions from the Small Intestine, Lung, and Lymph Nodes
  • 06:20Results: Flow Cytometry Analysis and Gating Strategy for the Identification of Th9 Cells and IL-9+ ILC2 Cells
  • 07:48Conclusion

Summary

Traduzione automatica

表达IL-9的T和ILC2细胞在 巴西猪笼草 感染期间被诱导,但由于它们的低频率和差异动力学,它们的表征在感染的肠道中在很大程度上被忽视了。该协议描述了从不同靶器官中分离这些细胞,并通过不同感染阶段的流式细胞术 确认 其身份。

Video correlati

Read Article