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A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model
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JoVE Journal 免疫学と感染
A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model

A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model

DOI:
10.3791/64075-v

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08:38 min

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March 03, 2023

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, , , , ,
1Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

章

  • 00:05Introduction
  • 01:03Isolation of Mediastinal Lymph Nodes, Lungs, Mesenteric Lymph Node Chains, and Small Intestine
  • 02:37Preparation of Single-Cell Suspensions from the Small Intestine, Lung, and Lymph Nodes
  • 06:20Results: Flow Cytometry Analysis and Gating Strategy for the Identification of Th9 Cells and IL-9+ ILC2 Cells
  • 07:48Conclusion

概要

自動翻訳

自動翻訳

IL-9-expressing T and ILC2 cells are induced during N. brasiliensis infection, yet their characterization has been largely overlooked in the infected intestine due to their low frequency and differential kinetics. This protocol describes the isolation of these cells from different target organs and confirmation of their identity via flow cytometry at different infection stages.

タグ

IL-9Nippostrongylus BrasiliensisLymphoid CellsInfection ModelIntracellular StainingCD4+ T CellsInnate Lymphoid CellsLungSmall IntestineLymph NodesLarvaeStandardization

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