/
/
A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model
1,108 Views
•
08:38 min
•
March 03, 2023
DOI:
Summary
Automatically generated
IL-9-expressing T and ILC2 cells are induced during N. brasiliensis infection, yet their characterization has been largely overlooked in the infected intestine due to their low frequency and differential kinetics. This protocol describes the isolation of these cells from different target organs and confirmation of their identity via flow cytometry at different infection stages.
Read Article
Cite this Article
Muñoz-Paleta, O., Olguín-Martínez, E., Ruiz-Medina, B. E., Alonso-Quintana, A., Marcial-Medina, M. C., Licona-Limón, P. A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model. J. Vis. Exp. (193), e64075, doi:10.3791/64075 (2023).
Copy