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Measuring Caenorhabditis elegans Sensitivity to the Acetylcholine Receptor Agonist Levamisole
Capitoli
- 00:05Introduction
- 00:45Preparation of Plates for the Levamisole Assay
- 02:03Synchronizing C. elegans
- 03:42Plating Synchronized C. elegans
- 04:36Performing the Levamisole Assay
- 05:47Data Analysis
- 06:52Results: Levamisole-Induced Paralysis in Wild Type and Representative Mutant C. elegans
- 07:28Conclusion
The present protocol describes an assay to determine response to levamisole, a pharmacological agonist of one class of Caenorhabditis elegans acetylcholine receptors. In this liquid levamisole swim assay, researchers visually observe and quantitate the time-dependent paralysis of animals cultivated in 24-well plates.
