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从肉鸡雏鸡胚胎中分离出前脂肪细胞
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation of Preadipocytes from Broiler Chick Embryos
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从肉鸡雏鸡胚胎中分离出前脂肪细胞

DOI:
10.3791/63861-v

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06:32 min

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August 04, 2022

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, , , ,
1Department of Animal Science,University of Tennessee

Capitoli

  • 00:05Introduction
  • 01:03Adipose Tissue Collection and Digestion
  • 02:21Digestion of Adipose Tissue
  • 04:48Results: Comparison of Cell Morphology and Lipid Accumulation
  • 05:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

本方案描述了一种从肉鸡胚胎中的脂肪组织中分离前脂肪细胞的简单方法。该方法能够高产率、原代培养和前脂肪细胞的脂肪分化进行分离。油红O染色和脂质/DNA染色测量了分化培养基诱导的分离细胞的脂肪生成能力。

Tags

Preadipocyte IsolationBroiler Chick EmbryosAdipocyte DifferentiationPrimary PreadipocytesEmbryonic Fat GrowthPoultry Research Model

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