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Isolatie van groep 2 aangeboren lymfoïde cellen van muis neusslijmvlies om de expressie van CD226 te detecteren
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
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Isolatie van groep 2 aangeboren lymfoïde cellen van muis neusslijmvlies om de expressie van CD226 te detecteren

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08:30 min

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May 10, 2022

DOI:

10.3791/63525-v

DOI:
10.3791/63525-v

•

08:30 min
May 10, 2022

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5 Views
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1Otolaryngological Department of Tangdu Hospital,Fourth Military Medical University, 2Institute of Medical Research,Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital,Fourth Military Medical University, 4Department of Immunology,Fourth Military Medical University

Summary

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Groep 2 aangeboren lymfoïde cellen (ILC2's), betrokken bij type 2-ontsteking, nemen voornamelijk deel aan de reactie op worminfectie, allergische aandoeningen, metabole homeostase en weefselherstel. In deze studie wordt een procedure aangetoond om ILC2's te isoleren van murine neusslijmvlies en de expressie van CD226 te detecteren.

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Xie, Y., Zhang, Y., Liu, Y., Wang, Y., Cheng, K., Zhuang, R., Bian, K. Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226. J. Vis. Exp. (183), e63525, doi:10.3791/63525 (2022).

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