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Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
JoVE Journal
Biologia
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JoVE Journal Biologia
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

DOI:
10.3791/63326-v

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10:53 min

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January 20, 2022

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, , ,
1Center for Cancer Biology,VIB (BE), 2Department of Oncology,KU Leuven (BE), 3Netherlands Cancer Institute, Department of Molecular Pathology,Oncode Institute (NL)

Capitoli

  • 00:05Introduction
  • 01:03Preparation of the R.MIW
  • 02:13R.MIW Implantation
  • 05:19Repeated Intravital Microscopy Through the R.MIW
  • 07:03Opening and Closing the Lid of the R.MIW in Between Imaging Sessions
  • 07:52Results: Multi-Day IVM of Mammary Gland Remodeling
  • 10:11Conclusion

Summary

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This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.

Tags

Mammary Imaging WindowLongitudinal Intravital MicroscopyMammary GlandMammary TumorSingle-cell ImagingCyanoacrylate AdhesiveGlass Cover SlipSurgical ProcedureRegion-of-interest

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