Journal
/
/
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
DOI:

10:53 min

January 20, 2022

, , ,

Chapitres

  • 00:05Introduction
  • 01:03Preparation of the R.MIW
  • 02:13R.MIW Implantation
  • 05:19Repeated Intravital Microscopy Through the R.MIW
  • 07:03Opening and Closing the Lid of the R.MIW in Between Imaging Sessions
  • 07:52Results: Multi-Day IVM of Mammary Gland Remodeling
  • 10:11Conclusion

Summary

Traduction automatique

This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.

Vidéos Connexes

Read Article