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Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
JoVE Revista
Biología
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JoVE Revista Biología
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

2,787 Views

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10:53 min

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January 20, 2022

DOI:

10.3791/63326-v

DOI:
10.3791/63326-v

•

10:53 min
January 20, 2022

•

14 Views
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1Center for Cancer Biology,VIB (BE), 2Department of Oncology,KU Leuven (BE), 3Netherlands Cancer Institute, Department of Molecular Pathology,Oncode Institute (NL)

Summary

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This protocol describes a novel mammary imaging window with a replaceable lid (R.MIW). Intravital microscopy after implantation of the R.MIW allows for longitudinal and multi-day imaging of the healthy and diseased mammary gland with a cellular resolution during the different developmental stages.

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Cite this Article

Mourao, L., Ciwinska, M., van Rheenen, J., Scheele, C. L. G. J. Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid. J. Vis. Exp. (179), e63326, doi:10.3791/63326 (2022).

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