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基于开放搜索的方法在 鲍曼不动杆菌 O-连锁糖肽鉴定中的应用
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JoVE Journal Biochimica
The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
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基于开放搜索的方法在 鲍曼不动杆菌 O-连锁糖肽鉴定中的应用

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08:37 min

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November 02, 2021

DOI:

10.3791/63242-v

DOI:
10.3791/63242-v

•

08:37 min
November 02, 2021

•

1 Views
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1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,The University of Melbourne

Summary

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开放式搜索能够鉴定用先前未知的聚糖组合物修饰的糖肽。在本文中，提出了一种简化的方法，用于进行开放搜索和随后以聚糖为重点的糖肽搜索，用于使用 鲍曼不动杆菌 作为模型的细菌样品。

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Acinetobacter BaumanniiO-linked GlycopeptidesOpen SearchingGlycosylationProteomeSTB-RPSZIC-HILICChromatographyEnrichmentMass Spectrometry

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Cite this Article

Lewis, J. M., Coulon, P. M. L., McDaniels, T. A., Scott, N. E. The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides. J. Vis. Exp. (177), e63242, doi:10.3791/63242 (2021).

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