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Differentiering af humane pluripotente stamceller til pancreas betacelleprækursorer i et 2D-kultursystem
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Capitoli
- 00:05Introduction
- 01:22Induction of Definitive Endoderm Differentiation in Human Pluripotent Stem Cells (hPSCs) (Stage 1)
- 02:10Immunofluorescence Analysis of hPSC-Derived Definitive Endoderm (Stage 1)
- 04:19Generation of the Primitive Gut Tube from hPSCs (Stage 2)
- 05:50Assessment of Differentiation Efficiency of Generating Pancreatic Progenitors
- 08:10Results: Comparison between Optimized and Non-Optimized Protocol for Scalable Generation of Pancreatic Progenitors from hPSCs
- 09:21Conclusion
Den nuværende protokol beskriver en forbedret metode til at øge co-ekspressionen af PDX1 og NKX6.1 transkriptionsfaktorer i bugspytkirtlen forfædre afledt af humane pluripotente stamceller (hPSC'er) i plane monolag. Dette opnås ved at genopfylde den friske matrix, manipulere celletæthed og dissociere de endodermale celler.
