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研究斑马鱼脊椎动物分割的三维尾部外植培养

JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

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研究斑马鱼脊椎动物分割的三维尾部外植培养

3,971 Views

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11:24 min

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June 30, 2021

DOI:

10.3791/61981-v

DOI:

10.3791/61981-v

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11:24 min

June 30, 2021

•

1 Views

M. Fethullah Simsek^1,3, Ertugrul M. Özbudak^1,2,3

¹Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, ²Department of Pediatrics,University of Cincinnati College of Medicine, ³Department of Genetics,Albert Einstein College of Medicine

Summary

在这里，我们介绍了斑马鱼后体轴的三维组织培养方案，从而能够对脊椎动物分割进行实时研究。此除草模型可控制轴伸长、形态源的改变以及细胞下分辨率组织水平的实时成像。

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