Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / TurboID-Based Proximity Labeling for In Planta…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
 

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Article DOI: 10.3791/60728-v • 07:02 min • May 17th, 2020
May 17th, 2020 •
*1, *2,3, 1, 1, 2, 2,3
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, 3Genome Center, College of Biological Sciences, University of California, Davis
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.

Tags

TurboID-based Proximity Labeling Protein-protein Interaction Networks NLR Immune Receptors Plant Defense Nicotiana Benthamiana Toll/interleukin-1 Receptor Domain Protein-protein Interactions Transient Interactions Weak Interactions In Vivo Labeling TurboID Fusions Agrobacterium Tumefaciens Competent Cells Agroinfiltration Abaxial Epidermis Biotin Labeling
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter