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A Method for Labeling Vasculature in Embryonic Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Method for Labeling Vasculature in Embryonic Mice

A Method for Labeling Vasculature in Embryonic Mice

15,425 Views

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09:58 min

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October 07, 2011

DOI:

10.3791/3267-v

DOI:
10.3791/3267-v

•

09:58 min
October 07, 2011

•

2 Views
, ,
1Department of Cellular Biology,University of Georgia, 2Centre for Immunology and Infection, Department of Biology and HYMS,University of York, 3Department of Genetics,University of Georgia

Summary

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This article describes a method for labeling embryonic skin and thymus blood vessels.

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Labeling VasculatureEmbryonic MiceBlood Vessel NetworkOrganogenesisThymusVasculature FormationThymocyte EntryMature T-cell ExitBlood Vessel DefectsFITC-dextranGriffonia Simplicifolia Lectin IGSL 1 – Isolectin B4CD31 Antibody StainingThymic Perivascular MesenchymeCryosectionsVibratome SectionsThymus Vascular DefectsTEC-derived MoleculesVascular NetworksMultiple Organs

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Bryson, J. L., Coles, M. C., Manley, N. R. A Method for Labeling Vasculature in Embryonic Mice. J. Vis. Exp. (56), e3267, doi:10.3791/3267 (2011).

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