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Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
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04:45 min
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February 10, 2022
DOI:
1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Chemistry,University of South Florida, 3Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign
Summary
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Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.
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Sparks, R. P., Lawless, W., Arango, A. S., Tajkhorshid, E., Fratti, R. A. Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions. J. Vis. Exp. (180), e60607, doi:10.3791/60607 (2022).
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