Journal
/
生物化学
/
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

DOI:
10.3791/60607-v

•

04:45 min

•

February 10, 2022

•

, , , ,
1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Chemistry,University of South Florida, 3Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign

Chapters

  • 00:04Introduction
  • 00:49Microscale Thermophoresis (MST) Device Preparation
  • 01:36Labeled MST Sample Preparation
  • 02:24Sample MST
  • 02:54Results: Representative Thermophoretic Traces
  • 03:46Conclusion

Summary

自动翻译

自动翻译

Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.

Tags

Keywords: Microscale ThermophoresisProtein-lipid InteractionsVam7Phosphatidic AcidDisassociation Constant (KD)Biochemical InteractionsNTA-Atto 647 DyeThermophoretic Traces1:1 TitrationDiC8 PAPharmaceutical Development

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/60607?article_type=v

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
check_url/cn/60607?article_type=v

Quantitative Immunofluorescence to Measure Global Localized Translation
check_url/cn/60607?article_type=v

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
check_url/cn/60607?article_type=v

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
check_url/cn/60607?article_type=v

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
check_url/cn/60607?article_type=v

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
check_url/cn/60607?article_type=v

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
check_url/cn/60607?article_type=v

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
check_url/cn/60607?article_type=v

CD Spectroscopy to Study DNA-Protein Interactions
check_url/cn/60607?article_type=v

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Read Article