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Biochemistry

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JoVE Journal Biochemistry
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
 

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Article DOI: 10.3791/60607-v • 04:45 min • February 10th, 2022
February 10th, 2022 •
*1, *2, *1,3,4, 1,3,4, 1,3
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Chemistry, University of South Florida, 3Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.

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Microscale Thermophoresis Protein-lipid Interactions Disassociation Constants KDs Biochemical Interactions Vam7 Phosphatidic Acid Membranes Protein Cost Pharmaceutical Development Lead Compounds MST Device Control Software Capillary Tube Target Ligand Ligand Analyte Concentration Titration Ratio MST Powers Binding Fit Labeled MST Samples Vam7-octahistidine Solution NTA-Atto 647 Dye
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