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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
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Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
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February 19, 2020
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Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.
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Pradhan, S. K., Guzman, J., Dargitz, C., Switalski, S., Landon, M., Olek, S., Samans, B., Hoffmueler, U., Lakshmipathy, U. Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR. J. Vis. Exp. (156), e60465, doi:10.3791/60465 (2020).
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