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Immunology and Infection

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JoVE Journal Immunology and Infection
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
 

Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR

Article DOI: 10.3791/60465-v • 08:02 min • February 19th, 2020
February 19th, 2020 •
1, 1, 1, 2, 1, 3, 3, 3, 1
1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine

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Resumen
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Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.

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Immune Cell Identity Immune Cell Purity Epigenetic-based Quantitative PCR Flow Cytometry Limitations Standardized Method Automated Data Analysis Template Assay Controls Genomic DNA Isolation Spectrophotometer Optical Density Dilution Calibrator Elution Buffer Reference Genomic DNA Incubation Bisulfite Conversion Ammonium Bisulfite THFA
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