This content is Free Access.
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
6,722 Views
•
08:02 min
•
February 19, 2020
DOI:
概要
Automatically generated
Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.
Read Article
Cite this Article
Pradhan, S. K., Guzman, J., Dargitz, C., Switalski, S., Landon, M., Olek, S., Samans, B., Hoffmueler, U., Lakshmipathy, U. Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR. J. Vis. Exp. (156), e60465, doi:10.3791/60465 (2020).
Copy