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Developmental Biology

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JoVE Journal Developmental Biology
Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
 

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Article DOI: 10.3791/60282-v • 08:25 min • June 2nd, 2020
June 2nd, 2020 •
1,2, 1
1Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, Parc Científic de Barcelona, 2The Francis Crick Institute

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This protocol is designed for the imaging and analysis of the dynamics of cell orientation and tissue growth in the Drosophila abdominal epithelia as the fruit fly undergoes metamorphosis. The methodology described here can be applied to the study of different developmental stages, tissues, and subcellular structures in Drosophila or other model organisms.

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Imaging Analysis Tissue Orientation Growth Dynamics Developing Drosophila Epithelia Pupal Stages Multicellular Organisms Spatial Orientation Cell Alignment Morphogenesis Planar Arrangements Development Cell Orientations Growth Dynamics Spacial Temporal Fidelity In Vivo Protocol Cell Behaviors Drosophila Pupal Epidermis Insights Research Theory Cell Biology Morphogenesis Planar Polarity Tissues Structures Models Precision Visualization Protocol Optimal Results Pupal Dissection
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