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可视化和分析星形细胞中细胞内器官和其他货物的细胞内传输
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
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可视化和分析星形细胞中细胞内器官和其他货物的细胞内传输

DOI:
10.3791/60230-v

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00:07 min

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August 28, 2019

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, ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill

Capitoli

  • 00:04Titolo
  • 00:31Fluorescently-Tagged Plasmid Transfection and Fluorescent Probe Late Endosome/Lysosome Labeling
  • 02:13Time-Lapse Imaging
  • 03:03Image Analysis
  • 05:00Results: Representative Quantitative Analyses of Cargo Dynamics in Astrocytes
  • 06:38Conclusion

Summary

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Traduzione automatica

在这里，我们描述了一种体外活成像方法，用于可视化细胞器的细胞内传输和在小鼠星形细胞中贩运血浆膜蛋白。该协议还提出了一种图像分析方法，以确定货物运输路线和动力学。

Tags

Intracellular TransportOrganelle MotilityAstrocytesLive ImagingFluorescent LabelingLipofectionTransfectionTime-lapse MicroscopyImage Analysis

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