Rivista
/
Immunologia e infezioni
/
慢性沙门氏菌感染诱发肠纤维化
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Chronic Salmonella Infection Induced Intestinal Fibrosis
Please note that all translations are automatically generated. Click here for the English version.

慢性沙门氏菌感染诱发肠纤维化

DOI:
10.3791/60068-v

•

08:40 min

•

September 22, 2019

•

, , ,
1The Biomedical Research Centre,University of British Columbia

Capitoli

  • 00:04Titolo
  • 01:30Preparation of Salmonella Typhimurium △AroA Cultures for Oral Gavage of Mice
  • 03:16Assessment of Salmonella Burdens in Tissues
  • 04:36Picrosirius Red Staining and Quantification of Collagen
  • 05:43Results: Chronic Salmonella Infection Induced Intestinal Fibrosis
  • 06:26Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了沙门氏菌驱动的肠道纤维化的小鼠模型，类似于克罗恩病的关键病理特征，包括跨体炎症和纤维化。该方法可用于评估使用维持在C57Bl/6遗传背景的突变小鼠改变纤维化结果的宿主因素。

Tags

Intestinal FibrosisChronic InflammationCrohn’s DiseaseSalmonella InfectionMouse ModelOral GavageStreptomycinLB AgarLB BrothBacterial CultureSterile Technique

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

长期慢性<em>铜绿假单胞菌</em>在呼吸道感染小鼠

鼠标型号病原体引起的慢性炎症局部及全身站点

一个协议感染<em>秀丽隐杆线虫</em>与<em>鼠伤寒沙门氏菌</em

胞浆的定量与液泡<em>沙门氏菌</em>在原发性巨噬细胞差透

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

利用人类诱导的多能干细胞衍生的肠道有机体研究和改性上皮细胞对沙门氏菌和其他病原体的保护

对TNBS介导肠纤维化发展的力学洞察与拉帕霉素抑制作用的评价

人类免疫缺陷小鼠模型中的慢性、急性和再激活HIV感染

慢性假单位病伤口感染的延迟接种模型

噬菌体纤维化 斑马鱼胚 胎中伪多多多阿韦诺萨感染的噬菌体治疗应用

Read Article