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Chronic Salmonella Infection Induced Intestinal Fibrosis
Chapters
- 00:04Title
- 01:30Preparation of Salmonella Typhimurium △AroA Cultures for Oral Gavage of Mice
- 03:16Assessment of Salmonella Burdens in Tissues
- 04:36Picrosirius Red Staining and Quantification of Collagen
- 05:43Results: Chronic Salmonella Infection Induced Intestinal Fibrosis
- 06:26Conclusion
This protocol describes a mouse model of Salmonella driven intestinal fibrosis that resembles key pathological hallmarks of Crohn’s disease including transmural inflammation and fibrosis. This method can be used to evaluate host factors that alter fibrotic outcomes using mutant mice maintained on a C57Bl/6 genetic background.
