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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
DOI:

08:00 min

October 18, 2019

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Capitoli

  • 00:04Titolo
  • 00:42Media Preparation and Sample Collection
  • 02:48TSC Sandwich Plating
  • 04:05Sub-culturing of Sulfite-Reducing Colonies
  • 05:08DNA Extraction and PCR Genotyping
  • 07:29Conclusion

Summary

Traduzione automatica

The objective of this protocol is to detect different Clostridium perfringens toxinotypes in locally purchased foods, particularly epsilon toxin producing strain types B and D, without the use of anaerobic chambers.

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