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JoVE Journal Environment
Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
 

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Article DOI: 10.3791/59931-v • 08:00 min • October 18th, 2019
October 18th, 2019 •
*1, *1, 1
1Brain and Mind Institute, Weill Cornell Medical College
* These authors contributed equally

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Resumen
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The objective of this protocol is to detect different Clostridium perfringens toxinotypes in locally purchased foods, particularly epsilon toxin producing strain types B and D, without the use of anaerobic chambers.

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Keywords: Clostridium Perfringens Toxinotypes Enrichment Detection Retail Food Samples Epsilon Toxin Modified Rapid Perfringens Medium (RPM) D-cycloserine Tryptose Sulfite Cycloserine (TSC) Agar Anaerobic Culture Spore Selection
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