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Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

DOI:
10.3791/59434-v

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06:31 min

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May 03, 2019

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, , ,
1Department of Systems Biology,Harvard Medical School

Capitoli

  • 00:04Titolo
  • 01:16Tool Preparation
  • 02:08Surgical Size Reduction and Recovery
  • 04:03Results: Surgical Size Reduction Leads to Changes in Morphology, Somite Size, and Neural Tube Heights
  • 05:24Conclusion

Summary

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Here, we describe a method for reducing the size of zebrafish embryos without disrupting normal developmental processes. This technique enables the study of pattern scaling and developmental robustness against size change.

Tags

ZebrafishEmbryonic Pattern ScalingSize ReductionLive ImagingGeneticaQuantitative AnalysisChorion RemovalWire LoopMethyl CelluloseRinger’s SolutionBlastulaAnimal Pole

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