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Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling
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JoVE Revista Biología del desarrollo
Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

DOI:
10.3791/59434-v

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06:31 min

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May 03, 2019

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, , ,
1Department of Systems Biology,Harvard Medical School

Capítulos

  • 00:04Título
  • 01:16Tool Preparation
  • 02:08Surgical Size Reduction and Recovery
  • 04:03Results: Surgical Size Reduction Leads to Changes in Morphology, Somite Size, and Neural Tube Heights
  • 05:24Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we describe a method for reducing the size of zebrafish embryos without disrupting normal developmental processes. This technique enables the study of pattern scaling and developmental robustness against size change.

Tags

ZebrafishEmbryonic Pattern ScalingSize ReductionLive ImagingGenéticaQuantitative AnalysisChorion RemovalWire LoopMethyl CelluloseRinger’s SolutionBlastulaAnimal Pole

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