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高盐抗原表达树突状细胞的分离和采用转移
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
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高盐抗原表达树突状细胞的分离和采用转移

5,468 Views

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09:29 min

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March 05, 2019

DOI:

10.3791/59124-v

DOI:
10.3791/59124-v

•

09:29 min
March 05, 2019

•

1 Views
, , , ,
1Division of Clinical Pharmacology, Department of Medicine,Vanderbilt University Medical Center

Summary

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在这里, 我们提出了一个协议, 以分离树突状细胞从小鼠的脾脏通过磁性细胞分类和随后采用转移到天真的小鼠。选择高盐活化树突状细胞模型, 解释采用转移和流式细胞术的分步过程。

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Cite this Article

Van Beusecum, J. P., Xiao, L., Barbaro, N. R., Patrick, D. M., Kirabo, A. Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells. J. Vis. Exp. (145), e59124, doi:10.3791/59124 (2019).

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