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1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Capitoli
- 00:04Titolo
- 00:36Preparation of the Gel
- 01:08Fixation of the Gel
- 01:40Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
- 02:41Fluorogenic Development of the Gel
- 04:05Destaining and Imaging
- 04:34Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
- 05:56Conclusion
Her beskriver vi en detaljeret protokol skitserer en ny fluorescerende farvning teknik til påvisning af total protein i polyacrylamidgeler. Protokollen udnytter en sølv ion-specifikke fluorescens turn-on sonde, som registrerer Ag+-proteinkomplekser, og fjerner visse begrænsninger af traditionelle kromogent sølv pletter.
