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Biochemistry

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JoVE Journal Biochemistry
Funktionelle karakterisering af Carboxylesterases i insekticid resistente stuefluer, Musca Domestica
 
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Funktionelle karakterisering af Carboxylesterases i insekticid resistente stuefluer, Musca Domestica

Article DOI: 10.3791/58106-v • 08:17 min • August 23rd, 2018
August 23rd, 2018 •
1, 1
1Department of Entomology and Plant Pathology, Auburn University

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Vi præsenterer her, en protokol for at producere hus flyve carboxylesterase proteiner i vitro med en baculovirus medieret insekt celle ekspressionssystem og senere funktionelt karakteriserer deres roller i metaboliske permethrin, dermed overdrage pyrethroid modstand af celle-baseret MTT assay og in vitro- metaboliske undersøgelser.

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Biokemi spørgsmålet 138 insekticidresistens carboxylesterases baculovirus-medieret insekt celle ekspressionssystem cytotoksicitet assay in vitro stofskifte analyse
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