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一种组合单细胞法表征人茎和祖种群的分子和免疫表型异质性
JoVE Journal
Genetica
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JoVE Journal Genetica
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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一种组合单细胞法表征人茎和祖种群的分子和免疫表型异质性

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09:34 min

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October 25, 2018

DOI:

10.3791/57831-v

DOI:
10.3791/57831-v

•

09:34 min
October 25, 2018

•

1 Views
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1Division of Molecular Hematology, Lund Stem Cell Center,Lund University

Summary

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大体积基因表达测量在异构细胞种群中云个体细胞差异。在这里, 我们描述了一个关于如何通过花期活化细胞分类 (immunophenotypically) 的单细胞基因表达分析和指数排序的协议, 以描绘异质性和分子不同细胞种群的特征。

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Cite this Article

Sommarin, M. N., Warfvinge, R., Safi, F., Karlsson, G. A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations. J. Vis. Exp. (140), e57831, doi:10.3791/57831 (2018).

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