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Deteção de evento raro usando o DNA de correção de erro e a sequenciação do ARN

JoVE Journal
Genetica

This content is Free Access.

JoVE Journal Genetica

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Deteção de evento raro usando o DNA de correção de erro e a sequenciação do ARN

11,545 Views

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10:36 min

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August 03, 2018

DOI:

10.3791/57509-v

DOI:

10.3791/57509-v

•

10:36 min

August 03, 2018

•

2 Views

Wing H. Wong*^1,2, R. Spencer Tong*^1,2, Andrew L. Young², Todd E. Druley²

¹Department of Pediatrics, Division of Hematology and Oncology,Washington University School of Medicine, ²Center for Genome Sciences and Systems Biology,Washington University School of Medicine

Tags

Rare Event Detection Error-corrected DNA Sequencing Error-corrected RNA Sequencing Clonal Single-nucleotide Variants Small Indels Unique Molecular Identifiers Q5 Master Mix PCR Magnetic Bead Cleanup Illumina Chemistry Gene Panels

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