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त्रुटि-सही डीएनए और आरएनए अनुक्रमण का उपयोग कर दुर्लभ घटना का पता लगाने
JoVE Journal
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JoVE Journal Genetica
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
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त्रुटि-सही डीएनए और आरएनए अनुक्रमण का उपयोग कर दुर्लभ घटना का पता लगाने

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10:36 min

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August 03, 2018

DOI:

10.3791/57509-v

DOI:
10.3791/57509-v

•

10:36 min
August 03, 2018

•

2 Views
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1Department of Pediatrics, Division of Hematology and Oncology,Washington University School of Medicine, 2Center for Genome Sciences and Systems Biology,Washington University School of Medicine

Tags

Rare Event DetectionError-corrected DNA SequencingError-corrected RNA SequencingClonal Single-nucleotide VariantsSmall IndelsUnique Molecular IdentifiersQ5 Master MixPCRMagnetic Bead CleanupIllumina ChemistryGene Panels

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Wong, W. H., Tong, R. S., Young, A. L., Druley, T. E. Rare Event Detection Using Error-corrected DNA and RNA Sequencing. J. Vis. Exp. (138), e57509, doi:10.3791/57509 (2018).

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