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线粒体钙在分离线粒体和培养细胞中的流入分析
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
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线粒体钙在分离线粒体和培养细胞中的流入分析

13,520 Views

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08:29 min

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April 27, 2018

DOI:

10.3791/57225-v

DOI:
10.3791/57225-v

•

08:29 min
April 27, 2018

•

12 Views
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1Department of Pediatrics,Emory University School of Medicine, 2Emory College of Arts and Sciences,Emory University

Summary

Automatically generated

在这里, 我们提出两个协议, 以测量线粒体 Ca2 +流入孤立的线粒体和培养细胞。对于孤立的线粒体, 我们使用 ca2 +敏感染料钙 green-5N, 详细介绍了一个基于板读取器的 ca2 +导入分析。对于培养细胞, 我们使用 Ca2 +染料 Rhod-2/AM 描述了共焦显微方法。

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Cite this Article

Maxwell, J. T., Tsai, C., Mohiuddin, T. A., Kwong, J. Q. Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells. J. Vis. Exp. (134), e57225, doi:10.3791/57225 (2018).

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