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人肠道 Organoids 上皮屏障通透性的实时测量
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
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人肠道 Organoids 上皮屏障通透性的实时测量

13,738 Views

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08:04 min

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December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

•

4 Views
, , , ,
1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

Summary

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本协议描述了在人体肠道 organoids 中采用荧光显微镜和活细胞显微镜进行药物治疗后 real-time 上皮屏障通透性的测量。

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Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

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