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Imaging del due-fotone calcio nei dendriti neuronali nelle fette del cervello
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Neuroscienze
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JoVE Journal Neuroscienze
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
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Imaging del due-fotone calcio nei dendriti neuronali nelle fette del cervello

10,251 Views

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10:35 min

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March 15, 2018

DOI:

10.3791/56776-v

DOI:
10.3791/56776-v

•

10:35 min
March 15, 2018

•

4 Views
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1CHU de Québec-Université Laval Research Center,Université Laval, 2Department of Biochemistry, Microbiology and Bioinformatics,Université Laval

Tags

Two-photon Calcium ImagingNeuronal DendritesBrain SlicesWhole-cell Patch-clampCalcium SignalingSynaptic InputsSpatiotemporal ResolutionACSF-sucroseVibratomeHippocampal SlicesACSF-recoveryTwo-photon MicroscopeInfrared Differential Interference Contrast MicroscopyStimulating PipettePatch PipetteMultiClamp AmplifierVoltage Clamp

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Cite this Article

Camiré, O., Topolnik, L. Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices. J. Vis. Exp. (133), e56776, doi:10.3791/56776 (2018).

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