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Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

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11:00 min

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November 09, 2017

DOI:

10.3791/56440-v

DOI:
10.3791/56440-v

•

11:00 min
November 09, 2017

•

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This protocol describes the quantification of multiple cytokine targets simultaneously in tissue culture supernatants collected from stimulated mouse splenocytes using multiplex bead based immunoassay platform and a flow cytometer.

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Cite this Article

Lehmann, J. S., Zhao, A., Sun, B., Jiang, W., Ji, S. Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform. J. Vis. Exp. (129), e56440, doi:10.3791/56440 (2017).

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