Journal
/
Inmunología y contagio
/
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

DOI:
10.3791/56440-v

•

11:00 min

•

November 09, 2017

•

, , , ,
1BioLegend

Capítulos

  • 00:05Título
  • 00:58Reagent and Standard Preparation
  • 02:57Assay Procedure
  • 06:23Flow Cytometer Set-up
  • 09:24Results: Quantification of Cytokines Produced by Mouse Splenocytes Cultured Under Stimulating Conditions
  • 10:15Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol describes the quantification of multiple cytokine targets simultaneously in tissue culture supernatants collected from stimulated mouse splenocytes using multiplex bead based immunoassay platform and a flow cytometer.

Tags

Multiplex Cytokine ProfilingMouse SplenocytesCytometric Bead-based ImmunoassayFlow CytometryCytokine QuantificationImmunologyELISAAntibody-immobilized BeadsMouse Th Cytokine StandardSerial DilutionAssay BufferFilter PlatesWash Buffer

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform

Antibiotic Dereplication Using the Antibiotic Resistance Platform

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Read Article