Journal
/
免疫与感染
/
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

44,189 Views

•

11:00 min

•

November 09, 2017

DOI:

10.3791/56440-v

DOI:
10.3791/56440-v

•

11:00 min
November 09, 2017

•

10 Views
, , , ,
1BioLegend

Summary

Automatically generated

This protocol describes the quantification of multiple cytokine targets simultaneously in tissue culture supernatants collected from stimulated mouse splenocytes using multiplex bead based immunoassay platform and a flow cytometer.

Explore More Videos

Multiplex Cytokine ProfilingMouse SplenocytesCytometric Bead-based ImmunoassayFlow CytometryCytokine QuantificationImmunologyELISAAntibody-immobilized BeadsMouse Th Cytokine StandardSerial DilutionAssay BufferFilter PlatesWash Buffer

Read Article

Cite this Article

Lehmann, J. S., Zhao, A., Sun, B., Jiang, W., Ji, S. Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform. J. Vis. Exp. (129), e56440, doi:10.3791/56440 (2017).

Download .ris file

Copy

Share Video

.

Copy