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Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

DOI:
10.3791/56218-v

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08:38 min

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October 30, 2017

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, ,
1Laboratory of Biochemical Genetics and Metabolism,The Rockefeller University

Capitoli

  • 00:05Titolo
  • 00:51In Vitro Chemotaxis
  • 02:56Murine Lymphocyte Adoptive Transfer
  • 05:17Collection and Analysis
  • 06:28Results: Representative In Vitro and In Vivo Migration Analyses
  • 07:44Conclusion

Summary

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Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.

Tags

Monocyte ChemotaxisIn VitroIn VivoLymphocyte TraffickingImmunologyTHP-1 CellsFluorescent LabelingCell MigrationMCP-1HEPESRPMI-1640C57BL/6 MouseAdoptive Transfer

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