需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
Chapters
- 00:05Title
- 00:51In Vitro Chemotaxis
- 02:56Murine Lymphocyte Adoptive Transfer
- 05:17Collection and Analysis
- 06:28Results: Representative In Vitro and In Vivo Migration Analyses
- 07:44Conclusion
Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.
