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Immunologie et infection
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Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
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Immunologie et infection
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Journal JoVE Immunologie et infection
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

DOI:
10.3791/56218-v

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08:38 min

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October 30, 2017

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, ,
1Laboratory of Biochemical Genetics and Metabolism,The Rockefeller University

Chapitres

  • 00:05Titre
  • 00:51In Vitro Chemotaxis
  • 02:56Murine Lymphocyte Adoptive Transfer
  • 05:17Collection and Analysis
  • 06:28Results: Representative In Vitro and In Vivo Migration Analyses
  • 07:44Conclusion

Summary

Traduction automatique

Traduction automatique

Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.

Tags

Monocyte ChemotaxisIn VitroIn VivoLymphocyte TraffickingImmunologyTHP-1 CellsFluorescent LabelingCell MigrationMCP-1HEPESRPMI-1640C57BL/6 MouseAdoptive Transfer

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