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Génération des variantes d’évasion de neutraliser les anticorps monoclonaux Influenza Virus
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
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Génération des variantes d’évasion de neutraliser les anticorps monoclonaux Influenza Virus

11,266 Views

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07:55 min

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August 29, 2017

DOI:

10.3791/56067-v

DOI:
10.3791/56067-v

•

07:55 min
August 29, 2017

•

3 Views
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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

Summary

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Les auteurs décrivent une méthode par laquelle nous identifions des résidus importants requis pour la liaison des anticorps monoclonaux humains ou murins qui ciblent l’hémagglutinine virale du virus de l’influenza. Le protocole peut être adapté à d’autres glycoprotéines de surface de virus et de leurs anticorps neutralisants correspondants.

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Cite this Article

Leon, P. E., Wohlbold, T. J., He, W., Bailey, M. J., Henry, C. J., Wilson, P. C., Krammer, F., Tan, G. S. Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies. J. Vis. Exp. (126), e56067, doi:10.3791/56067 (2017).

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