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質量分析を用いた識別 RNA 結合領域のための試料作製

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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質量分析を用いた識別 RNA 結合領域のための試料作製

7,951 Views

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10:52 min

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September 28, 2017

DOI:

10.3791/56004-v

DOI:

10.3791/56004-v

•

10:52 min

September 28, 2017

•

2 Views

Robert Warneford-Thomson^1,4, Chongsheng He^1,2, Simone Sidoli^1,3, Benjamin A. Garcia^1,3, Roberto Bonasio^1,2

¹Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, ²Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, ³Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, ⁴Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine

Summary

RNA 結合蛋白質を識別し、紫外線による光架橋と質量分析法を用いた生細胞におけるその RNA 結合領域をマップするためのプロトコルについて述べる。

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