Rivista
/
Immunologia e infezioni
/
基于磁珠复合法的撕裂细胞因子分析
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Please note that all translations are automatically generated. Click here for the English version.

基于磁珠复合法的撕裂细胞因子分析

DOI:
10.3791/55993-v

•

09:22 min

•

October 13, 2017

•

, , , , , ,
1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Capitoli

  • 00:05Titolo
  • 00:42Elution of Tear Sample from Tear Flow Strip
  • 01:26Cytokine Profiling by Bead Based Multiplex Assay
  • 04:43Reading the Assay Plate and Data Analysis
  • 06:56Results: Tear Cytokine Profiles from Four Male Subjects
  • 08:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

泪膜细胞因子的分析有助于研究各种眼部疾病。基于微珠的复合检测方法简单、灵敏, 能够对小体积样品中的多个目标进行测试。在这里, 我们描述了一个协议的撕裂膜细胞因子分析一个使用珠基复用法。

Tags

Tear Cytokine ProfileBead-based Multiplex AssaySchirmer StripOphthalmologyCytokinesOcular DiseasesAnalytesTear SampleAssay BufferCentrifugationCytokine ProfilingMultiplex AssayCytokine StandardsAssay WorksheetMagnetic Plate Washer

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

??PCR?????????????????????MEC??????V<em>???????</em

细胞因子产生的病毒逃避解剖先天免疫信号

高通量实时定量RT-PCR检测方法的确定I型和III型干扰素亚型的表达谱

巨细胞病毒特异性的自动化细胞富集的T细胞用于使用细胞因子捕获系统的临床应用

流式细胞仪为基础的检测方法的NK细胞功能的监控

基于荧光的淋巴细胞检测适用于小分子的高通量筛选

荧光为基础的神经氨酸酶抑制测定流感病毒的易感性评估抗病毒药的神经氨酸酶抑制剂类

细胞 Bead-based 免疫分析平台对受激小鼠脾多重细胞因子的研究

大鼠外周全血细胞免疫表型和因子生成的单细胞分析

通过流细胞学测定，测量自然获得的噬菌体诱导恶性疟Plasmodium falciparum原虫抗体

Read Article