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Imaging Subcellular Structures in the Living Zebrafish Embryo
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11:19 min
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April 02, 2016
DOI:
1Institute of Neuronal Cell Biology,Technische Universität München, 2Cell Biology, Department of Biology, Faculty of Science,Utrecht University, 3Faculty of Biology,Ludwig-Maximilians-Universität-München, 4Adolf-Butenandt-Institute, Biochemistry,Ludwig-Maximilians-Universität-München, 5German Center for Neurodegenerative Diseases, 6Laboratory of Brain Development and Repair,The Rockefeller University
Summary
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Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.
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Engerer, P., Plucinska, G., Thong, R., Trovò, L., Paquet, D., Godinho, L. Imaging Subcellular Structures in the Living Zebrafish Embryo. J. Vis. Exp. (110), e53456, doi:10.3791/53456 (2016).
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