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利用 Immunolabeling 分析斑马鱼胚胎中的稳定、动态和初生的微管

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

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利用 Immunolabeling 分析斑马鱼胚胎中的稳定、动态和初生的微管

DOI:

10.3791/55792-v

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12:38 min

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September 20, 2017

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Rebecca J. McFarland*¹, Sharlene P. Brown*¹, Eudorah Vital, Jonathan M. Werner, Rachel M. Brewster

¹Department of Biological Sciences,University of Maryland, Baltimore County

Capitoli

00:05Titolo
00:45Fixation of Staged Embryos
01:52Sectioning for Immunolabeling
04:04Confocal Imaging
05:17Quantifying Microtubules
07:10De Novo MT Assembly Assay
09:05Depolymerize Existing MTs
10:26Results: Analysis of Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
12:00Conclusion

Summary

Traduzione automatica

Immunolabeling 的方法来分析不同群体的微管在发展斑马鱼大脑中描述, 这是广泛适用于其他组织。第一个协议概述了 immunolabeling 稳定和动态微管的优化方法。第二个协议提供了一种具体的图像和量化初生微管的方法。

Tags

Keywords: Immunolabeling Microtubules Zebrafish Embryo Developmental Neurobiology Cell Polarity Stable Microtubules Dynamic Microtubules Nascent Microtubules Fixation Sectioning Immunostaining Primary Antibodies Secondary Antibodies DAPI

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